This research is supported by the National Institute On Drug Abuse of the National Institutes of Health (NIH) under Award Number U01DA053941. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

This work was supported financially by the University of Miami (Coral Gables, FL) administration, with in-kind contributions from University Facilities, University Environmental Health and Safety, and University of Miami Health Safety Division. Laboratory facilities and support were made available in-kind through the Sylvester Comprehensive Cancer Center, the Miami Center for AIDS Research, and the Miami Clinical and Translational Science Institute. Later parts of the research reported in this publication were supported by the National Institute On Drug Abuse of the National Institutes of Health (NIH) under Award Number U01DA053941.

The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. We are also thankful to our many colleagues with whom we have interacted and discussed best practices for wastewater sample collection, concentration, and analysis for SARSCoV- 2.

This project is supported by:

• National Institutes of Health (NIH) Rapid Acceleration of Diagnostics – Radical (RADx-rad) grant #1U01DA0539491-01
• NIH/NCI Cancer Center Support Grant #1P30CA240139-01 for the Sylvester Comprehensive Cancer Center
• NIH grant #P30AI073961 for the Miami Center for AIDS Research (CFAR), with the following NIH co-funding institutes and centers:
  • NIAID National Institute of Allergy and Infectious Diseases
  • NCI National Cancer Institute
  • NICHD Eunice Kennedy Shriver National Institute of Child Health and Human Development
  • NHLBI National Heart, Lung, and Blood Institute
  • NIDA National Institute on Drug Abuse
  • NIMHD National Institute on Minority Health and Health Disparities
  • NIA National Institute on Aging
  • NIDDK National Institute of Diabetes and Digestive and Kidney Diseases
  • NIDCR National Institute of Dental and Craniofacial Research
  • NIMH National Institute of Mental Health
  • NINR National Institute of Nursing Research
  • NIGMS National Institute of General Medical Sciences
  • NIH OD Office of the Director
• NIH grant #1UL1TR000460 for the University of Miami Clinical and Translational Science Institute (CTSI), with the following NIH co-funding institutes and centers: NCATS (National Center for Advancing Translational Sciences) and NIMHD
• University of Miami internal funding for wastewater surveillance at the University
• University of Miami College of Engineering for provision of space and access to vehicles to conduct field sampling
• University of Miami Environmental Health and Safety for provision of sampling support and expertise in disinfection strategies
• University of Miami Facilities (Gables and Miller School of Medicine) for sampling support and access to sewer systems
• University of Miami Housing and IT for provision of student housing information and hospital statistics
• Miami-Dade Water and Sewer Department for access to municipal wastewater and laboratory access during weekly and hourly sampling
• Florida Department of Health in Miami-Dade County

The MetaSUB Consortium, established by Weill Cornell Medicine, is supported by crowdfunding and by industry partners, including Copan, CosmosID, GISCloud, IBM, Illumina, OneCodex, Promega, QIAGEN, Isohelix, and Zymo Research.

The project’s contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH.

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